Don't think they apply for this do they? It's the EFL trophy which has the numerical requirements.
I can't see anything in the regulations about how many players from the previous matchday squad have to be included in the cup squads, it just says "full strength".
There are a few other sources that say it's 7 from the previous squad need to be in the squad, so theoretically you could make wholesale changes as long as they're deemed to be of a suitable strength.